Contact an Account ManagerChromatography of tryptic peptides of recombinant human growth hormone
Summary
Type: HPLC
Application #: 108080
Catalog#: 720120.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x150mm
Detection: UV, 214 nm
Conditions: temperature 35 °C
eluent: 5min isocratic 0.12% aqueous trifluoroacetic acid (TFA), then linear gradient to 38% acetonitrile in 60min, finally gradient to 57% acetonitrile over 10min
flow rate 1ml/min
Authors: Frenz J
Source: J Chromatogr 512 (1990) 299 - 314 and J Chromatogr
Details
Legend: Fig. 2. Tryptic map of 200µg of a biosynthetic human growth hormone digest
mixture.
Displacement chromatography for the isolation of minor components from the
digest mixture is performed on two